Helichrysum gymnocephalum Essential Oil: Chemical Composition and Cytotoxic, Antimalarial and Antioxidant Activities, Attribution of the Activity Origin by Correlations

被引:49
作者
Afoulous, Samia [1 ]
Ferhout, Hicham [2 ]
Raoelison, Emmanuel Guy [3 ]
Valentin, Alexis [4 ]
Moukarzel, Beatrice [4 ]
Couderc, Francois [1 ]
Bouajila, Jalloul [1 ]
机构
[1] Univ Toulouse 3, Lab Interact Mol & React Chim & Photochim UMR CNR, Univ Toulouse, F-31062 Toulouse, France
[2] NatEx Biotech, F-31400 Toulouse, France
[3] IMRA, Lab Phytochim & Standardisat, Antananarivo 101, Madagascar
[4] Univ Toulouse 3, Fac Pharm, Univ Toulouse, UMR IRD UPS Pharma DEV 152, F-31062 Toulouse 9, France
关键词
Helichrysum gymnocephalum; GC-MS; antimalarial activity; cytotoxic activity; antioxidant activity; LEAF ESSENTIAL OIL; IN-VITRO; VOLATILE OIL; ANTIPROLIFERATIVE ACTIVITY; BIOLOGICAL-ACTIVITIES; ERYTHROCYTIC STAGES; BIOACTIVITY; EXTRACTS; LEAVES;
D O I
10.3390/molecules16108273
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Helichrysum gymnocephalum essential oil (EO) was prepared by hydrodistillation of its leaves and characterized by GC-MS and quantified by GC-FID. Twenty three compounds were identified. 1,8-Cineole (47.4%), bicyclosesquiphellandrene (5.6%), gamma-curcumene (5.6%), alpha-amorphene (5.1%) and bicyclogermacrene (5%) were the main components. Our results confirmed the important chemical variability of H. gymnocephalum. The essential oil was tested in vitro for cytotoxic (on human breast cancer cells MCF-7), antimalarial (Plasmodium falciparum: FcB1-Columbia strain, chloroquine-resistant) and antioxidant (ABTS and DPPH assays) activities. H. gymnocephalum EO was found to be active against MCF-7 cells, with an IC50 of 16 +/- 2 mg/L. The essential oil was active against P. falciparum (IC50 = 25 +/- 1 mg/L). However, the essential oil exhibited a poor antioxidant activity in the DPPH (IC50 value > 1,000 mg/L) and ABTS (IC50 value = 1,487.67 +/- 47.70 mg/L) assays. We have reviewed the existing results on the anticancer activity of essential oils on MCF-7 cell line and on their antiplasmodial activity against the P. falciparum. The aim was to establish correlations between the identified compounds and their biological activities (antiplasmodial and anticancer). beta-Selinene (R-2 = 0.76), alpha-terpinolene (R-2 = 0.88) and aromadendrene (R-2 = 0.90) presented a higher relationship with the anti-cancer activity. However, only calamenene (R-2 = 0.70) showed a significant correlation for the antiplasmodial activity.
引用
收藏
页码:8273 / 8291
页数:19
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