Genetic and physical analysis of a YAC contig spanning the fungal disease resistance locus Asc of tomato (Lycopersicon esculentum)

被引：16

作者：

Mesbah, LA ^{[1
]}

Kneppers, TJA ^{[1
]}

Takken, FLW ^{[1
]}

Laurent, P ^{[1
]}

Hille, J ^{[1
]}

Nijkamp, HJJ ^{[1
]}

机构：

[1] Free Univ Amsterdam, Bioctr Amsterdam, Inst Mol Biol Sci, Dept Genet, NL-1081 HV Amsterdam, Netherlands

来源：

MOLECULAR AND GENERAL GENETICS | 1999年 / 261卷 / 01期

关键词：

tomato; Alternaria stem canker; map-based cloning; physical mapping; recombination;

D O I：

10.1007/s004380050940

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Alternaria stem canker disease of tomato is caused by the necrotrophic fungal pathogen Alternaria alternata f. sp. lycopersici (AAL). The fungus produces AAL toxins that kill the plant tissue. Resistance to the fungus segregates as a single locus, called Asc, and has been genetically mapped on chromosome 3 of tomato. We describe here the establishment of a 1383-kb YAC; contig covering the Ase locus and a series of plants selected for recombination events around the Ase locus. It was shown that the YAC contig corresponds to a genetic distance of at least 11.2 cM. Thus, the recombination rate in the Ase region is six times higher (123 kb/cM) than the average for the tomato genome. Furthermore, the Ase locus could be localised to a 91-kb fragment, thus paving the way for the cloning and identification of the Ase gene(s) by complementation.

引用

页码：50 / 57

页数：8

共 40 条

[1]

Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P211, DOI DOI 10.1007/BF02672069

[2]

Bent AF, 1996, PLANT CELL, V8, P1757, DOI 10.1105/tpc.8.10.1757

[3] An improved method of partially digesting plant megabase DNA suitable for YAC cloning: Application to the construction of a 5.5 genome equivalent YAC library of tomato [J].

Bonnema, G ;

Hontelez, J ;

Verkerk, R ;

Zhang, YQ ;

vanDaelen, R ;

vanKammen, A ;

Zabel, P .

PLANT JOURNAL, 1996, 9 (01) :125-133

[4]

Brandwagt B, 1998, DEV PLANT PATHOL, V13, P317

[5] Map-based cloning of the tomato genomic region that spans the Sw-5 tospovirus resistance gene in tomato [J].

Brommonschenkel, SH ;

Tanksley, SD .

MOLECULAR & GENERAL GENETICS, 1997, 256 (02) :121-126

[6] CLONING OF LARGE SEGMENTS OF EXOGENOUS DNA INTO YEAST BY MEANS OF ARTIFICIAL CHROMOSOME VECTORS [J].

BURKE, DT ;

CARLE, GF ;

OLSON, MV .

SCIENCE, 1987, 236 (4803) :806-812

[7] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[8] THE RELATIONSHIP BETWEEN GENETIC AND PHYSICAL DISTANCES IN THE CLONED A1-SH2 INTERVAL OF THE ZEA-MAYS L GENOME [J].

CIVARDI, L ;

XIA, YJ ;

EDWARDS, KJ ;

SCHNABLE, PS ;

NIKOLAU, BJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (17) :8268-8272

[9]

Dellaporta S.L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI DOI 10.1007/BF02712670

[10] A 13-KILODALTON MAIZE MITOCHONDRIAL PROTEIN IN ESCHERICHIA-COLI CONFERS SENSITIVITY TO BIPOLARIS-MAYDIS TOXIN [J].

DEWEY, RE ;

SIEDOW, JN ;

TIMOTHY, DH ;

LEVINGS, CS .

SCIENCE, 1988, 239 (4837) :293-295

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