Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

被引：80

作者：

Chumbimuni-Torres, Karin Y. ^{[1
]}

Coronado, Ramon E. ^{[2
]}

Mfuh, Adelphe M. ^{[1
]}

Castro-Guerrero, Carlos ^{[3
]}

Silva, Maria Fernanda ^{[4
]}

Negrete, George R. ^{[1
]}

Bizios, Rena ^{[2
]}

Garcia, Carlos D. ^{[1
]}

机构：

[1] Univ Texas San Antonio, Dept Chem, San Antonio, TX 78249 USA

[2] Univ Texas San Antonio, Dept Biomed Engn, San Antonio, TX 78249 USA

[3] Univ Texas San Antonio, Dept Phys & Astron, San Antonio, TX 78249 USA

[4] Natl Univ Cuyo, Sch Agron Sci, IBAM, CONICET, Mendoza, Argentina

来源：

RSC ADVANCES | 2011年 / 1卷 / 04期

基金：

美国国家卫生研究院;

关键词：

BOVINE SERUM-ALBUMIN; SPECTROSCOPIC ELLIPSOMETRY; CAPILLARY-ELECTROPHORESIS; SURFACE MODIFICATION; FIBRINOGEN ADSORPTION; MICROFLUIDIC DEVICES; RELAXATION KINETICS; OPTICAL-PROPERTIES; POLY(DIMETHYLSILOXANE); SILICA;

D O I：

10.1039/c1ra00198a

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.

引用

页码：706 / 714

页数：9

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