A distinct member of the aspartic proteinase gene family from the human malaria parasite Plasmodium falciparum

被引:61
作者
Berry, C
Humphreys, MJ
Matharu, P
Granger, R
Horrocks, P
Moon, RP
Certa, U
Ridley, RG
Bur, D
Kay, J
机构
[1] Cardiff Univ, Cardiff Sch Biosci, Cardiff CF1 3US, S Glam, Wales
[2] Univ Oxford, Inst Mol Med, Mol Parasitol Grp, Oxford OX3 9DS, England
[3] Pharma Res Pre Clin, Hoffmann La Roche Pharmaceut Div, Basel, Switzerland
关键词
malaria; aspartic proteinase; Plasmodium;
D O I
10.1016/S0014-5793(99)00276-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A gene (hap) transcribed during the intra-erythrocytic life cycle stages of the human malaria parasite Plasmodium falciparum was cloned and sequenced. It was found to encode a protein belonging to the aspartic proteinase family but which carried replacements of catalytically crucial residues in the hallmark sequences contributing to the active site of this type of proteinase, Consideration is given as to whether this protein is the first known parasite equivalent of the pregnancy-associated glycoproteins that have been documented in ungulate mammals. Alternatively, it may be operative as a new type of proteinase with a distinct catalytic mechanism. In this event, since no counterpart is known to exist in humans, it affords an attractive potential target against which to develop new anti-malarial drugs. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:149 / 154
页数:6
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