X-ray microanalysis of airway surface liquid collected in cystic fibrosis mice

被引:17
作者
Zahm, JM
Baconnais, S
Davidson, DJ
Webb, S
Dorin, J
Bonnet, N
Balossier, G
Puchelle, E
机构
[1] Inst Federatif Rech 53, INSERM, U514, F-51092 Reims, France
[2] Electron Microscopy Lab, Unite Format & Rech Sci, F-51685 Reims, France
[3] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
关键词
cryoprobe; ion concentration;
D O I
10.1152/ajplung.2001.281.2.L309
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The airway surface liquid (ASL) that lines the airway surface epithelium plays a major role in airway antibacterial defense and mucociliary transport efficiency, two key factors in cystic fibrosis (CF) disease. A major difficulty is to collect ASL in native conditions without stimulation or alteration of the underlying airway epithelium. Using a cryoprobe specifically adapted to collect native ASL from the tracheal mouse surface, we analyzed by X-ray microanalysis the complete ASL and plasma ion content in Cftr(tm1Hgu)/Cftr(tm1Hgu) mice compared with that in control littermates. ASL ion content from eight Cftr(tm1Hgu)/Cftr(tm1Hgu) mice and eight control littermates did not appear significantly different. The mean (+/- SE) concentrations were 2,352 +/- 367 and 2,058 +/- 401 mmol/kg dry weight for Na, 1,659 +/- 272 and 1,448 +/- 281 mmol/kg dry weight for Cl, 357 +/- 57 and 337 +/- 38 mmol/kg dry weight for S, 1,066 +/- 220 and 787 +/- 182 mmol/kg dry weight for K, 400 +/- 82 and 301 +/- 58 mmol/kg dry weight for Ca, 105 +/- 31 and 105 +/- 20 mmol/kg dry weight for Mg, 33 +/- 15 and 29 +/- 9 mmol/kg dry weight for P in non-CF and CF mice, respectively. This cryotechnique appears to be a promising technique for analyzing the complete elemental composition of native ASL in CF and non-CF tissues.
引用
收藏
页码:L309 / L313
页数:5
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