Labeling during cleavage (LDC), a new labeling approach for RNA

被引：6

作者：

Monnot, V ^{[1
]}

Tora, C ^{[1
]}

Lopez, S ^{[1
]}

Menou, L ^{[1
]}

Laayoun, A ^{[1
]}

机构：

[1] BioMerieux SA, Dept Mol Biol, F-69280 Marcy Letoile, France

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

关键词：

D O I：

10.1081/NCN-100002514

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new and efficient strategy for labeling of RNA sequences prior to their hybridization on high density DNA chip has been developed. Our approach which combines the fragmentation and the labeling is based on the reactivity of the 3'-phosphate of cleaved RNA fragments with a fluorescent molecule bearing aromatic bromomethyl function.

引用

页码：1177 / 1179

页数：3

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