Determination and phylogenetic analysis of partial sequences from TT virus isolates

被引:58
作者
Biagini, P
Gallian, P
Attoui, H
Cantaloube, JF
de Micco, P
de Lamballerie, X
机构
[1] Fac Med Marseille, Lab Virol Mol Trop & Transfus, F-13385 Marseille 05, France
[2] Etablissement Transfus Sanguine Alpes Provence, Mol Biol Lab, F-13392 Marseille, France
关键词
D O I
10.1099/0022-1317-80-2-419
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Sera from French in-patients were tested for the presence of the TT virus (TTV) genome using PCR and degenerate primers located in ORF1. Thirty-six sequences were determined and compared with those deposited in databases, revealing a high degree of genetic variability between TTV isolates (up to 47% for amino acid sequences). Phylogenetic analysis demonstrated the existence of three main groups corresponding to the previously described genotypes 1 and 2 and to a new genotype 3. Isolates could be assigned to distinct genotypes if their genetic distance was > 27%. No comparable genetic criteria were found for the definition of sub-types in the region studied. A 15-31 month follow-up of three haemodialysis patients proved the existence of chronic infection by TTV, In one patient, two strains belonging to different genotypes were detected at the same time. Sequences of both ORF1 and ORF2 remained unchanged for a given strain during the follow-up.
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收藏
页码:419 / 424
页数:6
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