Applications of DNA shuffling to pharmaceuticals and vaccines

被引：130

作者：

Patten, PA

Howard, RJ

Stemmer, WPC

机构：

[1] Maxygen, Inc., 3410 Central Expressway, Santa Clara

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1997年 / 8卷 / 06期

关键词：

D O I：

10.1016/S0958-1669(97)80127-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA shuffling is a practical process for directed molecular evolution which uses recombination to dramatically accelerate the rate at which one can evolve genes. Single and multigene traits that require many mutations for improved phenotypes can be evolved rapidly. DNA shuffling technology has been significantly enhanced in the past year, extending its range of applications to small molecule pharmaceuticals, pharmaceutical proteins, gene therapy vehicles and transgenes, vaccines and evolved viruses for vaccines, and laboratory animal models. (C) Current Biology Ltd ISSN 0958-1669.

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页码：724 / 733

页数：10

相关论文

共 32 条

[1]

ARNOLD FH, 1997, ADV BIOCH ENG BIOTEC, V58, P2

[2] ISOLATION OF NEW RIBOZYMES FROM A LARGE POOL OF RANDOM SEQUENCES [J].

BARTEL, DP ;

SZOSTAK, JW .

SCIENCE, 1993, 261 (5127) :1411-1418

[3] Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5 [J].

Bergelson, JM ;

Cunningham, JA ;

Droguett, G ;

KurtJones, EA ;

Krithivas, A ;

Hong, JS ;

Horwitz, MS ;

Crowell, RL ;

Finberg, RW .

SCIENCE, 1997, 275 (5304) :1320-1323

[4] EMERGENCE OF A REPLICATING SPECIES FROM AN IN-VITRO RNA EVOLUTION REACTION [J].

BREAKER, RR ;

JOYCE, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :6093-6097

[5]

BURBANK L, 1914, L BURBANK HIS METHOD, V1, P176

[6]

CALDWELL RC, 1992, PCR METH APPL, V2, P28

[7] Chimeric proteins can exceed the sum of their parts: Implications for evolution and protein design [J].

Campbell, RK ;

Bergert, ER ;

Wang, YH ;

Morris, JC ;

Moyle, WR .

NATURE BIOTECHNOLOGY, 1997, 15 (05) :439-443

[8] Improved green fluorescent protein by molecular evolution using DNA shuffling [J].

Crameri, A ;

Whitehorn, EA ;

Tate, E ;

Stemmer, WPC .

NATURE BIOTECHNOLOGY, 1996, 14 (03) :315-319

[9] Construction and evolution of antibody-phage libraries by DNA shuffling [J].

Crameri, A ;

Cwirla, S ;

Stemmer, WPC .

NATURE MEDICINE, 1996, 2 (01) :100-102

[10] Molecular evolution of an arsenate detoxification pathway DNA shuffling [J].

Crameri, A ;

Dawes, G ;

Rodriguez, E ;

Silver, S ;

Stemmer, WPC .

NATURE BIOTECHNOLOGY, 1997, 15 (05) :436-438

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