In vivo fiber-optic confocal reflectance microscope with an injection-molded plastic miniature objective lens

被引:96
作者
Carlson, K
Chidley, M
Sung, KB
Descour, M
Gillenwater, A
Follen, M
Richards-Kortum, R
机构
[1] Univ Texas, Dept Biomed Engn, Austin, TX 78712 USA
[2] Univ Arizona, Ctr Opt Sci, Tucson, AZ 85721 USA
[3] Univ Texas, MD Anderson Canc Ctr, Houston, TX 77030 USA
关键词
D O I
10.1364/AO.44.001792
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
For in vivo optical diagnostic technologies to be distributed to the developed and developing worlds, optical imaging systems must be constructed of inexpensive components. We present a fiber-optic confocal reflectance microscope with a cost-effective injection-molded plastic miniature objective lens for in vivo imaging of human tissues in near real time. The measured lateral resolution is less than 2.2 mu m, and the measured axial resolution is 10 mu m. Confocal images of ex vivo cervical tissue biopsies and in vivo human lip taken at 15 frames/s demonstrate the microscope's capability of imaging cell morphology and tissue architecture. (c) 2005 Optical Society of America.
引用
收藏
页码:1792 / 1797
页数:6
相关论文
共 22 条
[1]  
[Anonymous], 2002, NAT CANC CONTR PROGR, V2nd
[2]   Cost-effectiveness analysis of diagnosis and management of cervical squamous intraepithelial lesions [J].
Cantor, SB ;
Mitchell, MF ;
Tortolero-Luna, G ;
Bratka, CS ;
Bodurka, DC ;
Richards-Kortum, R .
OBSTETRICS AND GYNECOLOGY, 1998, 91 (02) :270-277
[3]  
CAVANAGH H D, 1990, CLAO Journal, V16, P65
[4]   Miniature injection-molded optics for fiber-optic, in vivo confocal microscopy [J].
Chidley, MD ;
Liang, C ;
Descour, MR ;
Sung, KB ;
Richards-Kortum, R ;
Gillenwater, AM .
INTERNATIONAL OPTICAL DESIGN CONFERENCE 2002, 2002, 4832 :126-136
[5]  
CHIDLEY MD, 1630, OPTICAL SCI CTR U AR
[6]   Near real-time confocal microscopy of amelanotic tissue: Detection of dysplasia in ex vivo cervical tissue [J].
Collier, T ;
Lacy, A ;
Richards-Kortum, R ;
Malpica, A ;
Follen, M .
ACADEMIC RADIOLOGY, 2002, 9 (05) :504-512
[7]   Silicon-micromachined scanning confocal optical microscope [J].
Dickensheets, DL ;
Kino, GS .
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 1998, 7 (01) :38-47
[8]  
Ferlay J., 2001, GLOBOCAN 2000 CANC I
[9]   CONFOCAL MICROSCOPY THROUGH A FIBEROPTIC IMAGING BUNDLE [J].
GMITRO, AF ;
AZIZ, D .
OPTICS LETTERS, 1993, 18 (08) :565-567
[10]   IMAGE-FORMATION IN A FIBEROPTIC CONFOCAL SCANNING MICROSCOPE [J].
GU, M ;
SHEPPARD, CJR ;
GAN, X .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1991, 8 (11) :1755-1761