Antileukoprotease in human skin: An antibiotic peptide constitutively produced by keratinocytes

被引：142

作者：

Wiedow, O ^{[1
]}

Harder, J ^{[1
]}

Bartels, J ^{[1
]}

Streit, V ^{[1
]}

Christophers, E ^{[1
]}

机构：

[1] Univ Kiel, Dept Dermatol, D-24105 Kiel, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 03期

关键词：

secretory leukocyte protease inhibitor; mucus proteinase inhibitor; antimicrobial peptides;

D O I：

10.1006/bbrc.1998.9069

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antileukoprotease (ALP), also known as mucous protease inhibitor or secretory leukoprotease inhibitor, resembles one of the major antiproteases present in human body fluids. It is capable of preventing proteolytic degradation of extracellular matrix proteins by neutrophil-derived serine proteases. ALP was isolated from human callus and detected in supernatants of cultured human primary keratinocytes. ALP mRNA was constitutively expressed in keratinocytes and the expression was not significantly affected by TNF alpha or Interferon gamma stimulation. In microbicidal assays recombinant ALP exhibited antimicrobial activity against several human skin associated microorganisms like P. aeruginosa, S. aureus, S. epidermidis, and C. albicans, indicating that ALP may actively participate in mechanisms allowing homeostasis of bacterial and yeast colonization on human skin. Thus, ALP represents a major soluble serine protease inhibitor and antimicrobial agent expressed in human skin and seems to contribute to the high resistance of the epidermis against proteolysis and infections. (C) 1998 Academic Press.

引用

页码：904 / 909

页数：6

共 22 条

[1] PREGNANCY-ASSOCIATED ENDOMETRIAL EXPRESSION OF ANTILEUKOPROTEINASE GENE IS CORRELATED WITH EPITHELIOCHORIAL PLACENTATION [J].