A lab-on-a-chip for rapid blood separation and quantification of hematocrit and serum analytes

被引：57

作者：

Browne, Andrew W. ^{[1
,2
,3
,4
]}

Ramasamy, Lakshminarayanan ^{[1
]}

Cripe, Timothy P. ^{[3
,4
]}

Ahn, Chong H. ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Elect & Comp Engn, Microsyst & BioMEMS Lab, Cincinnati, OH 45221 USA

[2] Univ Cincinnati, Coll Med, Phys Scientist Training Program, Cincinnati, OH 45267 USA

[3] Cincinnati Childrens Hosp, Med Ctr, Div Hematol Oncol, Cincinnati, OH USA

[4] Cincinnati Childrens Hosp, Med Ctr, Div Expt Hematol & Canc Biol, Cincinnati, OH USA

来源：

LAB ON A CHIP | 2011年 / 11卷 / 14期

关键词：

ELECTROKINETICALLY-CONTROLLED IMMUNOASSAY; CARDIAC TROPONIN-T; WHOLE-BLOOD; MICROFLUIDIC BIOCHIP; PLASMA; EXTRACTION; POLYMER; DESIGN; FLOW;

D O I：

10.1039/c1lc20144a

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

In this work, a new lab-on-a-chip for rapid analysis of low volume blood samples was designed, fabricated and demonstrated for integration of serum separation, hematocrit evaluation, and protein quantitation. Blood separation was achieved using microchannel flow-based separation. A novel method for evaluating hematocrit from microfluidic flow-separated blood samples was developed using gray scale analysis of a point-and-shoot digital photograph of separated blood in a micochannel. Protein quantitation was subsequently performed in a high surface area-to-volume ratio microfluidic chemiluminescent immunoassay using cell depleted serum produced by microfluidic flow-based separation of whole blood samples. All three steps were achieved in a single microchannel with separation of blood samples and hematocrit evaluation in less than 1 min, and protein quantitation in 5 min.

引用

页码：2440 / 2446

页数：7

共 43 条

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Role of serum cardiac troponin T in the diagnosis of acute rheumatic fever and rheumatic carditis [J].