Species Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Systems

被引:83
作者
Justesen, Ulrik Stenz [1 ]
Holm, Anette [2 ]
Knudsen, Elisa [1 ]
Andersen, Line Bisgaard [2 ]
Jensen, Thoger Gorm [1 ]
Kemp, Michael [1 ]
Skov, Marianne Nielsine [1 ]
Gahrn-Hansen, Bente [1 ]
Moller, Jens Kjolseth [2 ]
机构
[1] Odense Univ Hosp, Dept Clin Microbiol, DK-5000 Odense C, Denmark
[2] Vejle Hosp, Dept Clin Microbiol, Vejle, Denmark
关键词
ROUTINE IDENTIFICATION; REVOLUTION; LABORATORIES;
D O I
10.1128/JCM.05788-11
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We compared two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria (n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria.
引用
收藏
页码:4314 / 4318
页数:5
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