Mortalization of human promyelocytic leukemia HL-60 cells to be more susceptible to sodium butyrate induced apoptosis and inhibition of telomerase activity by down-regulation of nucleophosmin/B23

被引:50
作者
Liu, WH [1 ]
Yung, BYM [1 ]
机构
[1] Chang Gung Univ, Coll Med, Dept Pharmacol, Canc Biochem Lab, Tao Yuan 333, Taiwan
关键词
nucleophosmin/B23; apoptosis; telomerase activity;
D O I
10.1038/sj.onc.1202234
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Vanadate (10 mu M), a potent inhibitor of tyrosine phosphatase, added simultaneously potentiated BuONa-induced (1 mM) apoptosis, The steady-state level of nucleophosmin/B23 mRNA and the total cellular nucleophosmin/B23 protein decreased during the BuONa/vanadate-induced apoptosis. Stabilization and promotor transcriptional activity assays indicate that the decrease in nucleophosmin/B23 mRNA in BuONa/vanadate-treated HL-60 cells was transcriptionally regulated. A decline in telomerase activity was observed in HL-60 cells treated with BuONa/vanadate for 24-96 h. There was virtually no decline of nucleophosmin/B23 mRNA nor the telomerase activities during the growth arrest by serum-starvation. The decrease in nucleophosmin/B23 mRNA expression and telomerase activity in HL-60 cells subsequent to BuONa/vanadate treatment can thus be attributed to cellular apoptosis rather than the growth arrest induced by BuONa/vanadate. Nucleophosmin/BW antisense oligomer treatment significantly potentiated BuONa-induced apoptosis and inhibition of telomerase activity. Results of this study suggest that nucleophosmin/B23 is one of the key elements in the down-regulation of nucleolar function for cellular apoptosis and mortalization.
引用
收藏
页码:3055 / 3064
页数:10
相关论文
共 46 条
[1]  
Albanell J, 1996, CANCER RES, V56, P1503
[2]   TELOMERE LENGTH PREDICTS REPLICATIVE CAPACITY OF HUMAN FIBROBLASTS [J].
ALLSOPP, RC ;
VAZIRI, H ;
PATTERSON, C ;
GOLDSTEIN, S ;
YOUNGLAI, EV ;
FUTCHER, AB ;
GREIDER, CW ;
HARLEY, CB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10114-10118
[3]  
BERNHARD W, 1968, ULTRASTRUCT BIOL, V3, P81
[4]  
Bestilny LJ, 1996, CANCER RES, V56, P3796
[5]  
BUSCH H, 1963, CANCER RES, V23, P313
[6]   BUTYRIC-ACID AND ITS MONOSACCHARIDE ESTER INDUCE APOPTOSIS IN THE HL-60 CELL-LINE [J].
CALABRESSE, C ;
VENTURINI, L ;
RONCO, G ;
VILLA, P ;
CHOMIENNE, C ;
BELPOMME, D .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 195 (01) :31-38
[7]  
CHAN PK, 1989, CANCER RES, V49, P3271
[8]   CHARACTERIZATION OF THE CDNA-ENCODING HUMAN NUCLEOPHOSMIN AND STUDIES OF ITS ROLE IN NORMAL AND ABNORMAL GROWTH [J].
CHAN, WY ;
LIU, QR ;
BORJIGIN, J ;
BUSCH, H ;
RENNERT, OM ;
TEASE, LA ;
CHAN, PK .
BIOCHEMISTRY, 1989, 28 (03) :1033-1039
[9]   Potentiation of sodium butyrate-induced apoptosis by vanadate in human promyelocytic leukemia cell line HL-60 [J].
Chang, ST ;
Yung, BYM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 221 (03) :594-601
[10]   ONE-HOUR DOWNWARD ALKALINE CAPILLARY TRANSFER FOR BLOTTING OF DNA AND RNA [J].
CHOMCZYNSKI, P .
ANALYTICAL BIOCHEMISTRY, 1992, 201 (01) :134-139