Differential targeting of β-adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae -: A mechanism to functionally regulate the cAMP signaling pathway

Differential modes for beta (1)- and beta (2)-adrenergic receptor (AR) regulation of adenylyl cyclase in cardiomyocytes is most consistent with spatial regulation in microdomains of the plasma membrane. This study examines whether caveolae represent specialized subdomains that concentrate and organize these moieties in cardiomyocytes. Caveolae from quiescent rat ventricular cardiomyocytes are highly enriched in beta (2)-ARs, G alpha (i), protein kinase A RII alpha subunits, caveolin-3, and flotillins (caveolin functional homologues); beta (1)-ARs, m(2)-muscarinic cholinergic receptors, G alpha (s), and cardiac types V/VI adenylyl cyclase distribute between caveolae and other cell fractions, whereas protein kinase A RI alpha subunits, G protein-coupled receptor kinase-a, and clathrin are largely excluded from caveolae, Cell surface beta (2)-ARs localize to caveolae in cardiomyocytes and cardiac fibroblasts (with markedly different beta (2)-AR expression levels), indicating that the fidelity of beta (2)-AR targeting to caveolae is maintained over a physiologic range of beta (2)-AR expression. In cardiomyocytes, agonist stimulation leads to a marked decline in the abundance of beta (2)-ARs (but not beta (1)-ARs) in caveolae. Other studies show co-immunoprecipitation of cardiomyocytes adenylyl cyclase V/VI and caveolin-3, suggesting their in vivo association. However, caveolin is not required for adenylyl cyclase targeting to low density membranes, since adenylyl cyclase targets to low buoyant density membrane fractions of HEK cells that lack prototypical caveolins, Nevertheless, cholesterol depletion with cyclodextrin augments agonist-stimulated cAMP accumulation, indicating that caveolae function as negative regulators of cAMP accumulation. The inhibitory interaction between caveolae and the cAMP signaling pathway as well as domain-specific differences in the stoichiometry of individual elements in the beta -AR signaling cascade represent important modifiers of cAMP dependent signaling in the heart.