Polymorphisms in gyrA and gyrB subsp paratuberculosis genes among mycobacterium avium type I, II, and III isolates

被引：45

作者：

Castellanos, E.

Aranaz, A.

Romero, B.

de Juan, L.

Alvarez, J.

Bezos, J.

Rodriguez, S.

Stevenson, K.

Mateos, A.

Domínguez, L.

机构：

[1] Univ Complutense Madrid, Fac Vet, Dept Sanidad Anim, Lab Vigilance Sanitaria Vet, E-28040 Madrid, Spain

[2] Moredun Res Inst, Penicuik EH26 0PZ, Midlothian, Scotland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 10期

关键词：

D O I：

10.1128/JCM.01411-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.

引用

页码：3439 / 3442

页数：4

共 30 条

[1] Modeling bacterial evolution with comparative-genome-based marker systems:: Application to Mycobacterium tuberculosis evolution and pathogenesis [J].

Alland, D ;

Whittam, TS ;

Murray, MB ;

Cave, MD ;

Hazbon, MH ;

Dix, K ;

Kokoris, M ;

Duesterhoeft, A ;

Eisen, JA ;

Fraser, CM ;

Fleischmann, RD .

JOURNAL OF BACTERIOLOGY, 2003, 185 (11) :3392-3399

[2] Elevation of Mycobacterium tuberculosis subsp. caprae Aranaz et al. 1999 to species rank as Mycobacterium caprae comb. nov., sp. nov. [J].

Aranaz, A ;

Cousins, D ;

Mateos, A ;

Domínguez, L .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2003, 53 :1785-1789

[3] Silent nucleotide polymorphisms and a phyogeny for Mycobacterium tuberculosis [J].

Baker, L ;

Brown, T ;

Maiden, MC ;

Drobniewski, F .

EMERGING INFECTIOUS DISEASES, 2004, 10 (09) :1568-1577

[4] A new evolutionary scenario for the Mycobacterium tuberculosis complex [J].

Brosch, R ;

Gordon, SV ;

Marmiesse, M ;

Brodin, P ;

Buchrieser, C ;

Eiglmeier, K ;

Garnier, T ;

Gutierrez, C ;

Hewinson, G ;

Kremer, K ;

Parsons, LM ;

Pym, AS ;

Samper, S ;

van Soolingen, D ;

Cole, ST .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (06) :3684-3689

[5] Nested PCR on blood and milk for the detection of Mycobacterium avium subsp paratuberculosis DNA in clinical and subclinical bovine paratuberculosis [J].

Buergelt, CD ;

Williams, JE .

AUSTRALIAN VETERINARY JOURNAL, 2004, 82 (08) :497-503

[6] Detection and verification of Mycobacterium avium subsp paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn's disease [J].

Bull, TJ ;

McMinn, EJ ;

Sidi-Boumedine, K ;

Skull, A ;

Durkin, D ;

Neild, P ;

Rhodes, G ;

Pickup, R ;

Hermon-Taylor, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) :2915-2923

[7] Mycobacterium avium subsp paratuberculosis strains from cattle and sheep can be distinguished by a PCR test based on a novel DNA sequence difference [J].

Collins, DM ;

De Zoete, M ;

Cavaignac, SM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) :4760-4762

[8] Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle [J].

de Juan, L. ;

Alvarez, J. ;

Aranaz, A. ;

Rodriguez, A. ;

Romero, B. ;

Bezos, J. ;

Mateos, A. ;

Dominguez, L. .

VETERINARY MICROBIOLOGY, 2006, 115 (1-3) :102-110

[9] Genetic diversity of Mycobacterium avium subspecies paratuberculosis isolates from goats detected by pulsed-field gel electrophoresis [J].

de Juan, L ;

Mateos, A ;

Domínguez, L ;

Sharp, JM ;

Stevenson, K .

VETERINARY MICROBIOLOGY, 2005, 106 (3-4) :249-257

[10] Comparison of four different culture media for isolation and growth of type II and type I/III Mycobacterium avium subsp paratuberculosis strains isolated from cattle and goats [J].

de Juan, Lucia ;

Alvarez, Julio ;

Romero, Beatriz ;

Bezos, Javier ;

Castellanos, Elena ;

Aranaz, Alicia ;

Mateos, Ana ;

Dominguez, Lucas .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (09) :5927-5932

← 1 2 3 →