Characterization of NovP and NovN:: Completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring

被引：52

作者：

Meyers, CLF

Oberthür, M

Xu, H

Heide, L

Kahne, D

Walsh, CT

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[2] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

[3] Univ Tubingen, Inst Pharmaceut, D-72076 Tubingen, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 01期

关键词：

antibiotics; biosynthesis; enzymes; novobiocin; transferases;

D O I：

10.1002/anie.200352626

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two steps forward: The glycosylated aminocoumarin antibiotic novobiocin (1) is produced by. Streptomyces spheroides and inhibits the bacterial type II topoisomerase DNA gyrase. The sugar-tailoring enzymes NovP and NovN that catalyze the final two steps in novobiocin biosynthesis were overproduced in Escherichia coli and characterized as a methyltransferase and carbamoyltransferase, respectively.

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收藏

页码：67 / 70

页数：4

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