Crystallization of the bovine ADP/ATP carrier is critically dependent upon the detergent-to-protein ratio

被引：22

作者：

Dahout-Gonzalez, C

Brandolin, G

Pebay-Peyroula, E

机构：

[1] Univ Grenoble 1, CEA Grenoble, Dept Reponse & Dynam Cellulaires, CNRS,CEA,UMR 5092,Lab Biochim & Biophys Syst Inte, F-38054 Grenoble 9, France

[2] Univ Grenoble 1, Inst Biol Struct, CNRS, CEA,UMR 5075, F-38027 Grenoble 1, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903020699

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ADP/ATP carrier is an integral membrane protein located in the mitochondrial inner membrane. It mediates the exchange of cytosolic ADP for ATP generated in the mitochondrial matrix. Here, the purification and crystallization of the bovine ADP/ATP carrier in complex with the inhibitor carboxyatractyloside in the presence of LAPAO, an aminoxide detergent, is reported. High-quality crystals were only obtained when excess detergent was removed to reach a controlled detergent-to-protein ratio.

引用

页码：2353 / 2355

页数：3

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