Meta and para effects in the ultrafast excited-state dynamics of the green fluorescent protein chromophores

被引：95

作者：

Solntsev, Kyril M. ^{[1
]}

Poizat, Olivier ^{[2
,3
]}

Dong, Jian ^{[1
]}

Rehault, Julien ^{[2
,3
]}

Lou, Yongbing ^{[4
]}

Burda, Clemens ^{[4
]}

Tolbert, Laren M. ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

[2] Univ Sci & Technol Lille, Ctr Etud & Rech Lasers & Applicat, Lab Spectrochim Infrarouge & Raman, UMR 8516, F-59655 Villeneuve Dascq, France

[3] Univ Sci & Technol Lille, Ctr Etud & Rech Lasers & Applicat, FR 2416, CNRS, F-59655 Villeneuve Dascq, France

[4] Case Western Reserve Univ, Ctr Chem Dynam & Nanomat Res, Dept Chem, Cleveland, OH 44106 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2008年 / 112卷 / 09期

关键词：

D O I：

10.1021/jp077707t

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Femtosecond transient absorption and fluorescence upconversion experiments have been performed to investigate the photoinduced dynamics of the meta isomer of the green fluorescent protein chromophore, m-HBDI, and its O-methylated derivative, m-MeOBDI, in various solvent mixtures at neutral, acidic, and basic pH. The para isomer, p-HBDI, and its O- and N-methylated derivatives, p-MeOBDI and p-HBDIMe+, were also studied for comparison. In all cases, fast quenching of the excited S, state by internal conversion (IQ to the ground state was observed. In the para compounds, IC, presumably promoted by the internal twisting, arises in < 1 ps. A similar process takes place in the meta compounds in nonaqueous solvents but with notably slower kinetics. In aqueous solutions, the meta compounds undergo ultrafast intermolecular excited-state proton transfer that competes with isomerization.

引用

页码：2700 / 2711

页数：12

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