Production and clinical development of a Hansenula polymorpha-derived PEGylated hirudin

被引：21

作者：

Avgerinos, GC

Turner, BG

Gorelick, KJ

Papendieck, A

Weydemann, U

Gellissen, G

机构：

[1] Abbott Biores Ctr, Worcester, MA 01605 USA

[2] Rhein Biotech GmbH, Dusseldorf, Germany

来源：

SEMINARS IN THROMBOSIS AND HEMOSTASIS | 2001年 / 27卷 / 04期

关键词：

hirudin; PEG-hirudin; Hansenula polymorpha; manufacturing; clinical development;

D O I：

10.1055/s-2001-16889

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This article describes the expression of the hirudin gene heterologously in the methylotrophic yeast Hansenula polymorpba, the establishment of an industrial-scale production process and the subsequent clinical development of polyethylene glycol (PEG)-hirudin. PEGylation increases the molecular weight of hirudin, thereby reducing its kidney filtration rate and immunogenicity and increasing its half-life in the circulation.

引用

收藏

页码：357 / 371

页数：15

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