Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region

被引：15

作者：

De Bruyne, A

Yera, H

Le Guerhier, F

Boireau, P

Dupouy-Camet, J

机构：

[1] Univ Paris 05, Trichinella Natl Reference Ctr, AP HP, Hop Cochin, F-75014 Paris, France

[2] INRA, AFSSA, ENVA, UMR BIPAR, F-94703 Maisons Alfort, France

来源：

VETERINARY PARASITOLOGY | 2005年 / 132卷 / 1-2期

关键词：

Trichinella; 5S rDNA; PCR; sequence;

D O I：

10.1016/j.vetpar.2005.05.026

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We developed a PCR-based assay using a single primer pair to amplify the 5S ribosomal DNA intergenic spacer region to identify Trichinella isolates. In our method, amplified products are directly sequenced on both strands and compared to GenBank sequences. Using this method, we were able to identify Trichinella spiralis, T britovi and T nativa. This method permits rapid species identification of Trichinella isolates; however, further evaluation is required before recommending this approach for routine use. (c) 2005 Elsevier B.V. All rights reserved.

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收藏

页码：57 / 61

页数：5

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