S100A8/A9, a key mediator for positive feedback growth stimulation of normal human keratinocytes

被引:107
作者
Nukui, Takamasa [1 ]
Ehama, Ritsuko [2 ]
Sakaguchi, Masakiyo [1 ]
Sonegawa, Hiroyuki [1 ]
Katagiri, Chika [2 ]
Hibino, Toshihiko [2 ]
Huh, Nam-Ho [1 ]
机构
[1] Okayama Univ, Dept Cell Biol, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
[2] Shiseido Life Sci Res Ctr, Kanazawa Ku, Yokohama, Kanagawa 2368643, Japan
关键词
S100; MRP; psoriasis; inflammation;
D O I
10.1002/jcb.21639
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
S100A8 and S100A9 are known to be up-regulated in hyperproliferative and psoriatic epidermis, but their function in epidermal keratinocytes remains largely unknown. Here we show that (1) S100A8 and S100A9 are secreted by cultured normal human keratinocytes (NHK) in a cytokine-dependent manner, (2) when applied to NHK, recombinant S100A8/A9 (a 1:1 mixture of S100A8 and S100A9) induced expression of a number of cytokine genes such as IL-8/CXCL8, CXCL1, CXCL2, CXCL3, CCL20, IL-6, and TNF alpha that are known to be up-regulated in psoriatic epidermis, (3)the S100A8/ A9-induced cytokines in turn enhanced production and secretion of S100A8 and S100A9 by NHK, and (4) S100A8 and S100A8/A9 stimulated the growth of NHK at a concentration as low as 1 ng/ml. These results indicate the presence of a positive feedback loop for growth stimulation involving S100A8/A9 and cytokines in human epidermal keratinocytes, implicating the relevance of the positive feedback loop to the etiology of hyperproliferative skin diseases, including psoriasis.
引用
收藏
页码:453 / 464
页数:12
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