Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation

被引：68

作者：

Byun, Mi Ran ^{[1
]}

Kim, A. Rum ^{[1
]}

Hwang, Jun-Ha ^{[1
]}

Sung, Mi Kyung ^{[1
]}

Lee, Yeon Kyung ^{[1
]}

Hwang, Buyng Su ^{[2
]}

Rho, Jung-Rae ^{[2
]}

Hwang, Eun Sook ^{[3
,4
,5
]}

Hong, Jeong-Ho ^{[1
,6
]}

机构：

[1] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea

[2] Kunsan Natl Univ, Dept Oceanog, Jeonbuk 573701, South Korea

[3] Ewha Womans Univ, Coll Pharm, Seoul 120750, South Korea

[4] Ewha Womans Univ, Div Life & Pharmaceut Sci, Seoul 120750, South Korea

[5] Ewha Womans Univ, Ctr Cell Signaling & Drug Discovery Res, Seoul 120750, South Korea

[6] Korea Univ, Coll Pharm, Chungnam 339700, South Korea

来源：

FEBS LETTERS | 2012年 / 586卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

Phorbaketal A; TAZ; Osteoblast; Differentiation; ERK; EPITHELIAL-MESENCHYMAL TRANSITION; TRANSCRIPTION FACTORS; SKELETAL DEVELOPMENT; BONE-FORMATION; COACTIVATOR; EXPRESSION; OSTEOPOROSIS; INTERACTS; ENHANCER; PATHWAY;

D O I：

10.1016/j.febslet.2012.03.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK. Structured summary of protein interactions: TAZ physically interacts with RUNX2 by pull down (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1086 / 1092

页数：7

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