Pas, a novel protein required for protein secretion and attaching and effacing activities of enterohemorrhagic Escherichia coli

被引:32
作者
Kresse, AU
Schulze, K
Deibel, C
Ebel, F
Rohde, M
Chakraborty, T
Guzmán, CA
机构
[1] GBF, Natl Res Ctr Biotechnol, Div Microbiol, D-38124 Braunschweig, Germany
[2] Justus Liebig Univ, Inst Med Microbiol, D-35392 Giessen, Germany
关键词
D O I
10.1128/JB.180.17.4370-4379.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Enterohemorrhagic Escherichia coli (EHEC) exhibits a pattern of localized adherence to host cells, with the formation of microcolonies, and induces a specific histopathological phenotype collectively known as the attaching and effacing lesion, The genes encoding the products responsible for this phenotype are located on a 35-kb pathogenicity island designated the locus of enterocyte effacement, which is also shared by enteropathogenic E. coli, We have identified an open reading frame (ORF) which is located upstream of the espA, espB, and espD genes on the complementary strand and which exhibits high homology to the genes spiB from Salmonella, yscD from Yersinia, and pscD from Pseudomonas. Localization studies showed that the encoded product is present in the cytoplasmic and inner membrane fractions of EHEC. The construction and characterization of a recombinant clone containing an in-frame deletion of this ORF demonstrated that the encoded product is a putative member of a type III system required for protein secretion, Disruption of this ORF, designated pns (protein associated with secretion), abolished the secretion of Esp proteins. The mutant adhered only poorly and lost its capacities to trigger attaching and effacing activity and to invade HeLa cells, These results demonstrate that Pas is a virulence-associated factor that plays an essential role in EHEC pathogenesis.
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页码:4370 / 4379
页数:10
相关论文
共 65 条
[1]  
Acheson DW, 1996, ASM NEWS, V62, P302
[2]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[3]   A NEW-GENERATION OF INFORMATION-RETRIEVAL TOOLS FOR BIOLOGISTS - THE EXAMPLE OF THE EXPASY WWW SERVER [J].
APPEL, RD ;
BAIROCH, A ;
HOCHSTRASSER, DF .
TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (06) :258-260
[4]   ELEVATION OF INTRACELLULAR FREE CALCIUM LEVELS IN HEP-2 CELLS INFECTED WITH ENTEROPATHOGENIC ESCHERICHIA-COLI [J].
BALDWIN, TJ ;
WARD, W ;
AITKEN, A ;
KNUTTON, S ;
WILLIAMS, PH .
INFECTION AND IMMUNITY, 1991, 59 (05) :1599-1604
[5]   Erwinia amylovora secretes harpin via a type III pathway and contains a homolog of yopN of Yersinia spp. [J].
Bogdanove, AJ ;
Wei, ZM ;
Zhao, LP ;
Beer, SV .
JOURNAL OF BACTERIOLOGY, 1996, 178 (06) :1720-1730
[6]   CURRENT CONCEPTS - ESCHERICHIA-COLI O157-H7 AND THE HEMOLYTIC-UREMIC SYNDROME [J].
BOYCE, TG ;
SWERDLOW, DL ;
GRIFFIN, PM .
NEW ENGLAND JOURNAL OF MEDICINE, 1995, 333 (06) :364-368
[7]   COLONIZATION FACTORS OF DIARRHEAGENIC ESCHERICHIA-COLI AND THEIR INTESTINAL RECEPTORS [J].
CASSELS, FJ ;
WOLF, MK .
JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1995, 15 (03) :214-226
[8]  
CLAROS MG, 1994, COMPUT APPL BIOSCI, V10, P685
[9]   EspE, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90 kDa protein [J].
Deibel, C ;
Krämer, S ;
Chakraborty, T ;
Ebel, F .
MOLECULAR MICROBIOLOGY, 1998, 28 (03) :463-474
[10]   ANALYSIS OF PSEUDOMONAS GENE-PRODUCTS USING LACIQ PTRP-LAC PLASMIDS AND TRANSPOSONS THAT CONFER CONDITIONAL PHENOTYPES [J].
DELORENZO, V ;
ELTIS, L ;
KESSLER, B ;
TIMMIS, KN .
GENE, 1993, 123 (01) :17-24