Supercritical fluids are superior media for catalysis by cross-linked enzyme microcrystals of subtilisin Carlsberg

被引：14

作者：

Fontes, N

Almeida, MC

Garcia, S

Peres, C

Partridge, J

Halling, PJ

Barreiros, S

机构：

[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2781901 Oeiras, Portugal

[2] Univ Strathclyde, Dept Pure & Appl Chem, Glasgow G1 1XL, Lanark, Scotland

来源：

BIOTECHNOLOGY PROGRESS | 2001年 / 17卷 / 02期

关键词：

D O I：

10.1021/bp000148m

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC-ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO2, especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.

引用

页码：355 / 358

页数：4

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