Neural differentiation of adipose-derived stem cells isolated from GFP transgenic mice

被引：111

作者：

Fujimura, J

Ogawa, R

Mizuno, H

Fukunaga, Y

Suzuki, H

机构：

[1] Nippon Med Coll, Dept Pharmacol, Tokyo 113, Japan

[2] Nippon Med Coll, Dept Plast & Reconstruct Surg, Tokyo 113, Japan

[3] Nippon Med Coll, Dept Pediat, Tokyo 113, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 333卷 / 01期

关键词：

adipose-derived stem cells; electron microscopy; green fluorescent protein transgenic mice; immunochemistry; mesenchymal stem cells; neural induction; tissue engineering;

D O I：

10.1016/j.bbrc.2005.05.096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice, we have recently reported that adipose-derived stromal cells (ASCs) could differentiate into mesenchymal lineages in vitro. In this study, we performed neural induction using ASCs from GFP transgenic mice and were able to induce these ASCs into neuronal and glial cell lineages. Most of the neurally induced cells showed bipolar or multipolar appearance morphologically and expressed neuronal markers. Electron microscopy revealed their neuronal morphology. Some cells also showed glial phenotypes, as shown immunocytochemically. The present study clearly shows that ASCs derived from GFP transgenic mice differentiate into neural lineages in vitro, suggesting that these cells might provide an ideal source for further neural stem cell research with possible therapeutic application for neurological disorders. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：116 / 121

页数：6

共 32 条

[1] In vitro differentiation of human processed lipoaspirate cells into early neural progenitors [J].