An estimate of large-scale sequencing accuracy

被引：18

作者：

Hill, F ^{[1
]}

Gemünd, C ^{[1
]}

Benes, V ^{[1
]}

Ansorge, W ^{[1
]}

Gibson, TJ ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

EMBO REPORTS | 2000年 / 1卷 / 01期

关键词：

D O I：

10.1093/embo-reports/kvd015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The accuracy of large-scale DNA sequencing is difficult to estimate without redundant effort. We have found that the mobile genetic element IS10, a component of the transposon Tn10, has contaminated a significant number of clones in the public databases, as a result of the use of the transposon in bacterial cloning strain construction. These contaminations need to be annotated as such. More positively, by defining the range of sequence variation in IS10, we have been able to determine that the rate of sequencing errors is very low, most likely surpassing the stated aim of one error or less in ten thousand bases.

引用

页码：29 / 31

页数：3

共 16 条

[1]

ALTSCHUL SF, 1992, NUCLEIC ACIDS RES, V25, P3389

[2] ACCURACY OF DNA-SEQUENCING - SHOULD THE SEQUENCE QUALITY BE MONITORED [J].