Evaluation of humoral and cell-mediated inducible immunity to Haemophilus ducreyi in an animal model of chancroid

被引:11
作者
Desjardins, M
Filion, LG
Robertson, S
Kobylinski, L
Cameron, DW
机构
[1] OTTAWA GEN HOSP,DEPT PATHOL,OTTAWA,ON K1H 8L6,CANADA
[2] UNIV OTTAWA,DEPT MICROBIOL & IMMUNOL,OTTAWA,ON,CANADA
[3] UNIV OTTAWA,DEPT MED,OTTAWA,ON,CANADA
关键词
D O I
10.1128/IAI.64.5.1778-1788.1996
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
To study the mechanisms of inducible immunity to Haemophilus ducreyi infection in the temperature-dependent rabbit model of chancroid, we conducted passive immunization experiments and characterized the inflammatory infiltrate of chancroidal lesions, Polyclonal immunoglobulin G was purified from immune sera raised against H. ducreyi 35000 whole-cell lysate or a pilus preparation and from naive control rabbits, Rabbits were passively immunized with 24 or 48 mg of purified polyclonal immunoglobulin G intravenously, followed 24 h after infusion by homologous titered infectious challenge, Despite titratable antibody, no significant difference in infection or disease was observed, We then evaluated the immunohistology of lesions produced by homologous-strain challenge in sham-immunized rabbits and those protectively vaccinated by pilus preparation immunization, Immunohistochemical stains for CD5 and CD4 T-lymphocyte markers were performed on lesion sections 4, 10, 15, and 21 days from infection, Lesions of pilus preparation vaccinees compared with those of controls had earlier infiltration with significantly more T lymphocytes (CD5(+)) and,vith a greater proportion of CD4(+) T lymphocytes at day 4 (33% +/- 5.5% versus 9.7% +/- 2%; P = 0.092), corroborating earlier sterilization (5.0 +/- 2 versus 13.7 +/- 0.71 days; P < 0.001) and lesion resolution, Intraepithelial challenge of pilus-vaccinated rabbits with 100 mu g of the pilus preparation alone produced indurated lesions within 48 h with lymphoid and plasmacytoid infiltration, edema, and extravasation of erythrocytes, We conclude that passive immunization may not confer a vaccine effect in this model and that active vaccination with a pilus preparation induces a delayed-type hypersensitivity skin test response and confers protection through cell-mediated immunity seen as an amplified lymphocytic infiltrate and accelerated maturation of the T-lymphocyte response.
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页码:1778 / 1788
页数:11
相关论文
共 34 条
[1]  
ABBAS AK, 1991, CELLULAR MOL IMMUNOL, P228
[3]   SEXUALLY-TRANSMITTED DISEASES AND HUMAN-IMMUNODEFICIENCY-VIRUS CONTROL IN MALAWI - A FIELD-STUDY OF GENITAL ULCER DISEASE [J].
BEHETS, FMT ;
LIOMBA, G ;
LULE, G ;
DALLABETTA, G ;
HOFFMAN, IF ;
HAMILTON, HA ;
MOENG, S ;
COHEN, MS .
JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (02) :451-455
[4]   CONDOM USE PREVENTS GENITAL ULCERS IN WOMEN WORKING AS PROSTITUTES - INFLUENCE OF HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION [J].
CAMERON, DW ;
NGUGI, EN ;
RONALD, AR ;
SIMONSEN, JN ;
BRADDICK, M ;
BOSIRE, M ;
KIMATA, J ;
KAMALA, J ;
NDINYAACHOLA, JO ;
WAIYAKI, PG ;
PLUMMER, FA .
SEXUALLY TRANSMITTED DISEASES, 1991, 18 (03) :188-191
[5]  
CAMERON DW, 1989, LANCET, V2, P403
[6]  
CAMERON DW, 1988, 4 INT C AIDS STOCKH
[7]  
*CAN PHARM ASS, 1995, COMP PHARM SPEC, P521
[8]   SERUM THERAPY REVISITED - ANIMAL-MODELS OF INFECTION AND DEVELOPMENT OF PASSIVE ANTIBODY THERAPY [J].
CASADEVALL, A ;
SCHARFF, MD .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (08) :1695-1702
[9]   AMINO ACID COMPOSITIONS OF HUMAN AND RABBIT GAMMA-GLOBULINS AND OF FRAGMENTS PRODUCED BY REDUCTION [J].
CRUMPTON, MJ ;
WILKINSON, JM .
BIOCHEMICAL JOURNAL, 1963, 88 (02) :228-&
[10]   INDUCIBLE IMMUNITY WITH A PILUS PREPARATION BOOSTER VACCINATION IN AN ANIMAL-MODEL OF HAEMOPHILUS-DUCREYI INFECTION AND DISEASE [J].
DESJARDINS, M ;
FILION, LG ;
ROBERTSON, S ;
CAMERON, DW .
INFECTION AND IMMUNITY, 1995, 63 (05) :2012-2020