Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8)

被引：98

作者：

Nishida, T

Nakanishi, T

Shimo, T

Asano, M

Hattori, T

Tamatani, T

Tezuka, K

Takigawa, M ^{[1
]}

机构：

[1] Okayama Univ, Sch Dent, Dept Biochem & Mol Dent, Okayama 7008525, Japan

[2] Japan Tobacco Inc, Pharmaceut Frontier Res Labs, Yokohama, Kanagawa 2360004, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 247卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.8895

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell Line, HCS-2/8. The binding of I-125-labeIed recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Rd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of I-12S-CTGF-receptor complex with an apparent molecular weight of 280 kDa, The I-125-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF, In addition, the I-125-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells. (C) 1998 Academic Press.

引用

页码：905 / 909

页数：5

共 23 条

[1] THE MODULAR ARCHITECTURE OF A NEW FAMILY OF GROWTH-REGULATORS RELATED TO CONNECTIVE-TISSUE GROWTH-FACTOR
BORK, P
[J]. FEBS LETTERS, 1993, 327 (02) : 125 - 130
[2] CONNECTIVE-TISSUE GROWTH-FACTOR - A CYSTEINE-RICH MITOGEN SECRETED BY HUMAN VASCULAR ENDOTHELIAL-CELLS IS RELATED TO THE SRC-INDUCED IMMEDIATE EARLY GENE-PRODUCT CEF-10
BRADHAM, DM
IGARASHI, A
POTTER, RL
GROTENDORST, GR
[J]. JOURNAL OF CELL BIOLOGY, 1991, 114 (06) : 1285 - 1294
[3] Purification and characterization of novel heparin-binding growth factors in uterine secretory fluids - Identification as heparin-regulated M-r 10,000 forms of connective tissue growth factor
Brigstock, DR
Steffen, CL
Kim, GY
Vegunta, RK
Diehl, JR
Harding, PA
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (32) : 20275 - 20282
[4] ENOMOTO M, 1992, BIOL REGULATION CHON, P321
[5] GLENN K, 1982, J BIOL CHEM, V257, P5172
[6] SIGNIFICANT CORRELATION BETWEEN CONNECTIVE-TISSUE GROWTH-FACTOR GENE-EXPRESSION AND SKIN SCLEROSIS IN TISSUE-SECTIONS FROM PATIENTS WITH SYSTEMIC-SCLEROSIS
IGARASHI, A
NASHIRO, K
KIKUCHI, K
SATO, S
IHN, H
GROTENDORST, GR
TAKEHARA, K
[J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1995, 105 (02) : 280 - 284
[7] REGULATION OF CONNECTIVE-TISSUE GROWTH-FACTOR GENE-EXPRESSION IN HUMAN SKIN FIBROBLASTS AND DURING WOUND REPAIR
IGARASHI, A
OKOCHI, H
BRADHAM, DM
GROTENDORST, GR
[J]. MOLECULAR BIOLOGY OF THE CELL, 1993, 4 (06) : 637 - 645
[8] GROWTH-REGULATION IN SCLERODERMA FIBROBLASTS - INCREASED RESPONSE TO TRANSFORMING GROWTH-FACTOR-BETA-1
KIKUCHI, K
KADONO, T
IHN, H
SATO, S
IGARASHI, A
NAKAGAWA, H
TAMAKI, K
TAKEHARA, K
[J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1995, 105 (01) : 128 - 132
[9] DEMONSTRATION OF RECEPTORS FOR EPIDERMAL GROWTH-FACTOR ON CULTURED RABBIT CHONDROCYTES AND REGULATION OF THEIR EXPRESSION BY VARIOUS GROWTH AND DIFFERENTIATION FACTORS
KINOSHITA, A
TAKIGAWA, M
SUZUKI, F
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 183 (01) : 14 - 20
[10] Kothapalli D, 1997, CELL GROWTH DIFFER, V8, P61

← 1 2 3 →