GOLDEN 2: A novel transcriptional regulator of cellular differentiation in the maize leaf

被引:187
作者
Hall, LN [1 ]
Rossini, L [1 ]
Cribb, L [1 ]
Langdale, JA [1 ]
机构
[1] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
关键词
D O I
10.1105/tpc.10.6.925
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
The differentiation of distinct cell types within the leaf is essential for normal plant development. We characterized previously a transposon-induced mutant of maize (bundle sheath defective1) that disrupts the differentiation of a single photosynthetic cell type in the leaf. In this study, we show that this mutation is allelic to golden2 (g2), a lesion first reported 70 years ago. We cloned G2 by using Suppressor-mutator as a molecular tag. The gene encodes a 2.2-kb transcript that is present throughout the wild-type leaf but is most abundant in C-4 leaf blade tissue. Gene sequence data showed the existence of a bipartite nuclear localization signal encoded by the first axon, and we determined that G2 reporter gene fusions are targeted to the nucleus in onion epidermal cells. Further sequence analysis indicated the presence of a novel motif within the deduced protein sequence that shares features with TEA DNA binding domains. Therefore, we propose that G2 acts as a novel transcriptional regulator of cellular differentiation in the maize leaf.
引用
收藏
页码:925 / 936
页数:12
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