Direct observation of thiol ester formation between an enzyme and an active site directed inhibitor by FT-IR

被引：6

作者：

Kawasaki, T

Fujioka, Y

Saito, K

Ohta, H

机构：

[1] KEIO UNIV, DEPT CHEM, KOHOKU KU, YOKOHAMA, KANAGAWA 223, JAPAN

[2] NIPPON STEEL CORP LTD, ADV MAT & TECHNOL RES LABS, NAKAHARA KU, KAWASAKI, KANAGAWA 211, JAPAN

来源：

CHEMISTRY LETTERS | 1996年 / 03期

关键词：

D O I：

10.1246/cl.1996.195

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

FT-IR measurement disclosed a formation of a thiol ester bond between a cysteine of arylmalonate decarboxylase and an active site directed inhibitor, alpha-bromophenylacetate, strongly suggesting that the enzyme initially activates the substrates in a similar mechanism.

引用

页码：195 / 196

页数：2

共 7 条

[1] BOYER PD, 1975, ENZYMES, V6
[2] *INFR SPECTR ATL A, 1991, INFR SPECTR ATL COAT, V1, P502
[3] A NOVEL ENZYMATIC DECARBOXYLATION PROCEEDS VIA A THIOL ESTER INTERMEDIATE
KAWASAKI, T
WATANABE, M
OHTA, H
[J]. BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1995, 68 (07) : 2017 - 2020
[4] NONRIBOSOMAL POLYPEPTIDE SYNTHESIS ON POLYENZYME TEMPLATES
LIPMANN, F
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1973, 6 (11) : 361 - 367
[5] CLONING AND HETEROLOGOUS EXPRESSION OF A NOVEL ARYLMALONATE DECARBOXYLASE GENE FROM ALCALIGENES-BRONCHISEPTICUS KU-1201
MIYAMOTO, K
OHTA, H
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1992, 38 (02) : 234 - 238
[6] STEREOCHEMISTRY OF ENZYME-CATALYZED DECARBOXYLATION OF ALPHA-METHYL-ALPHA-PHENYLMALONIC ACID
MIYAMOTO, K
TSUCHIYA, S
OHTA, H
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (15) : 6256 - 6257
[7] ENZYME-MEDIATED ASYMMETRIC DECARBOXYLATION OF DISUBSTITUTED MALONIC-ACIDS
MIYAMOTO, K
OHTA, H
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (10) : 4077 - 4078

← 1 →