Identity of human normal counterpart (MmTRA1b) of mouse leukemogenesis-associated gene (MmTRA1a) product as plasma membrane phospholipid scramblase and chromosome mapping of the human MmTRA1b phospholipid scramblase gene

被引:25
作者
Kasukabe, T [1 ]
Kobayashi, H
Kaneko, Y
Okabe-Kado, J
Honma, Y
机构
[1] Saitama Canc Ctr, Res Inst, Ina, Saitama 3620806, Japan
[2] Saitama Canc Ctr, Hematol Clin, Ina, Saitama 3620806, Japan
关键词
D O I
10.1006/bbrc.1998.9190
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We recently cloned a new leukemogenesis-associated gene MmTRA1a (Mm-1 cell derived transplantability-associated gene 1a, former name "TRA1") from a mouse leukemogenic and monocytic Mm-P cell cDNA library and also cloned its normal counterpart MmTRA1b (former name "NOR1") from a normal mouse kidney cDNA library, The mouse MmTRA1a is a truncated form of mouse MmTRA1b. Here we report the cloning of a cDNA (human MmTRA1b) homologous to the mouse MmTRA1b from a human monocytic U937 cell cDNA library. The human MmTRA1b cDNA predicts a peptide containing 318 amino acids with a calculated molecular weight of 35,047 Ha. The predicted human MmTRA1b protein sequence shared 78% amino acid identity with the mouse counterpart (328 amino acids). Both the human homologue and mouse MmTRA1b protein but not MmTRA1a protein possess a proline-rich domain at the N-terminal end. The human MmTRA1b gene was mapped to chromosome 3q23, Expression of the human homologue was increased during differentiation of U937 cells induced by most typical differentiation inducers. Moreover, predicted amino acid sequence analysis of human MmTRA1b cDNA revealed perfect identity with the human plasma membrane phospholipid scramblase that is required for transbilayer movement of membrane phospholipids. These results provide new information on the possible roles of MmTRA1b/phospholipid scramblase and the truncated MmTRA1a in the leukemogenesis and differentiation of monocytic leukemia cells. (C) 1998 Academic Press.
引用
收藏
页码:449 / 455
页数:7
相关论文
共 21 条
[1]   PROLINE-RICH SEQUENCES THAT BIND TO SRC HOMOLOGY-3 DOMAINS WITH INDIVIDUAL SPECIFICITIES [J].
ALEXANDROPOULOS, K ;
CHENG, GH ;
BALTIMORE, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (08) :3110-3114
[2]   Isolation of an erythrocyte membrane protein that mediates Ca2+-dependent transbilayer movement of phospholipid [J].
Basse, F ;
Stout, JG ;
Sims, PJ ;
Wiedmer, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (29) :17205-17210
[3]   In vivo detection and imaging of phosphatidylserine expression during programmed cell death [J].
Blankenberg, FG ;
Katsikis, PD ;
Tait, JF ;
Davis, RE ;
Naumovski, L ;
Ohtsuki, K ;
Kopiwoda, S ;
Abrams, MJ ;
Darkes, M ;
Robbins, RC ;
Maecker, HT ;
Strauss, HW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (11) :6349-6354
[4]   MODULAR BINDING DOMAINS IN SIGNAL-TRANSDUCTION PROTEINS [J].
COHEN, GB ;
REN, RB ;
BALTIMORE, D .
CELL, 1995, 80 (02) :237-248
[5]   AATYK: A novel tyrosine kinase induced during growth arrest and apoptosis of myeloid cells [J].
Gaozza, E ;
Baker, SJ ;
Vora, RK ;
Reddy, EP .
ONCOGENE, 1997, 15 (25) :3127-3135
[6]   Significant improvement in accuracy of multiple protein sequence alignments by iterative refinement as assessed by reference to structural alignments [J].
Gotoh, O .
JOURNAL OF MOLECULAR BIOLOGY, 1996, 264 (04) :823-838
[7]  
Kanatani Y, 1996, CELL GROWTH DIFFER, V7, P187
[8]   TRA1, a novel mRNA highly expressed in leukemogenic mouse monocytic sublines but not in nonleukemogenic sublines [J].
Kasukabe, T ;
OkabeKado, J ;
Honma, Y .
BLOOD, 1997, 89 (08) :2975-2985
[9]   SELECTION OF MOUSE MACROPHAGE-LIKE SUBLINES THAT DIFFER IN LEUKEMOGENIC POTENTIAL AND CHARACTERIZATION [J].
KASUKABE, T ;
HONMA, Y ;
HOZUMI, M .
JOURNAL OF CELLULAR PHYSIOLOGY, 1984, 118 (02) :105-112
[10]   SH2 AND SH3 DOMAINS - ELEMENTS THAT CONTROL INTERACTIONS OF CYTOPLASMIC SIGNALING PROTEINS [J].
KOCH, CA ;
ANDERSON, D ;
MORAN, MF ;
ELLIS, C ;
PAWSON, T .
SCIENCE, 1991, 252 (5006) :668-674