TRP-PLIK, a bifunctional protein with kinase and ion channel activities

被引：601

作者：

Runnels, LW

Yue, LX

Capham, DE

机构：

[1] Harvard Univ, Childresn Hosp,Sch Med, Howard Hughes Med Inst, Dept Cardiol, Boston, MA 02115 USA

[2] Harvard Univ, Childrens Hosp,Sch Med, Howard Hughes Med Inst, Dept Neurobiol, Boston, MA 02115 USA

来源：

SCIENCE | 2001年 / 291卷 / 5506期

关键词：

D O I：

10.1126/science.1058519

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the Long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing alpha -kinase domain was functional, Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function.

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页码：1043 / 1047

页数：5

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