Ultra-high resolution X-ray diffraction from crystals of the kinetic mutant of human carbonic anhydrase II, His 64 Ala, and its complexes with proton acceptor/donors.

被引:5
作者
Duda, D
Tu, CK
Silverman, DN
Kalb, AJ
Agbandje-McKenna, M
McKenna, R
机构
[1] Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA
[2] Univ Florida, Dept Pharmacol & Therapeut, Gainesville, FL 32610 USA
[3] Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel
关键词
D O I
10.2174/0929866013409751
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Crystals of human carbonic anhydrase II with a specific point mutation, His 64 to Ala, have been grown in a solution of ammonium sulfate in the presence of mercury chloride. The crystals appear in approximately two weeks and belong to the monoclinic space group P2(1), with unit cell parameters of a = 42.2 Angstrom, b = 41.4 Angstrom c = 71.9 Angstrom beta = 104.2 degrees and one carbonic anhydrase molecule in the asymmetric unit. The crystals diffract X-rays beyond 1.0 Angstrom resolution. These crystals, soaked with exogenous proton acceptor/donors, will be used in X-ray and neutron diffraction studies to map the fine water structure "proton wire" in the active site of carbonic anhydrase and to assign the intra- and intermolecular proton transfer pathway(s) from the zinc-bound water out to the bulk solvent.
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收藏
页码:63 / 67
页数:5
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