Possibilities of flow cytometric analysis for immunophenotypic characterization of canine lymphoma

被引：30

作者：

Culmsee, K ^{[1
]}

Simon, D ^{[1
]}

Mischke, R ^{[1
]}

Nolte, I ^{[1
]}

机构：

[1] Univ Hannover, Dept Small Anim Med, Sch Vet Med, D-30173 Hannover, Germany

来源：

JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE | 2001年 / 48卷 / 04期

关键词：

D O I：

10.1046/j.1439-0442.2001.00351.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 [兽医学];

摘要：

The purpose of the study here is to show the possibilities of flow cytometric analysis for immunophenotypic characterization of canine malignant lymphoma. Aspiration biopsies from lymph nodes of 30 dogs with multicentric lymphoma were stained by four dog-specific antibodies and three human-specific antibodies cross-reacting with canine lymphocytes, and analysed by flow cytometry. Twenty of the 30 lymphoma examined were characterized as B-cell-lymphoma and 10 as T-cell-lymphoma. Flow cytometry is a suitable method for the immunophenotypic analysis of canine lymphoma. Contrary to immunohistochemistry, no surgically produced lymph node biopsy is required and the results are available within a few hours.

引用

页码：199 / 206

页数：8

共 20 条

[1]

MONOCLONAL-ANTIBODIES THAT DEFINE CANINE HOMOLOGS OF HUMAN CD ANTIGENS - SUMMARY OF THE FIRST INTERNATIONAL CANINE LEUKOCYTE ANTIGEN WORKSHOP (CLAW) [J].

COBBOLD, S ;

METCALFE, S .

TISSUE ANTIGENS, 1994, 43 (03) :137-154

[2]

DORN CR, 1967, AM J VET RES, V28, P933

[3]

ECKHARDT R, 1991, LAB MED, V15, P563

[4]

IMMUNOHISTOCHEMICAL DETECTION OF CD3 ANTIGEN (PAN-T MARKER) IN CANINE LYMPHOMAS [J].

FERRER, L ;

FONDEVILA, D ;

RABANAL, R ;

TARRES, J ;

RAMIS, A .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1993, 5 (04) :616-620

[5]

Cytohistological and immunological classification of canine malignant lymphomas: Comparison with human non-Hodgkin's lymphomas [J].

FournelFleury, C ;

Magnol, JP ;

Bricaire, P ;

Marchal, T ;

Chabanne, L ;

Delverdier, A ;

Bryon, PA ;

Felman, P .

JOURNAL OF COMPARATIVE PATHOLOGY, 1997, 117 (01) :35-59

[6]

GREENLEE PG, 1990, CANCER-AM CANCER SOC, V66, P480, DOI 10.1002/1097-0142(19900801)66:3&lt

[7]

480::AID-CNCR2820660314&gt

[8]

3.0.CO

[9]

2-X

[10]

Blood cell markers [J].

Grindem, CB .

VETERINARY CLINICS OF NORTH AMERICA-SMALL ANIMAL PRACTICE, 1996, 26 (05) :1043-+

← 1 2 →