Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants

被引：62

作者：

Hui, EKW

Wang, PC

Lo, SJ ^{[1
]}

机构：

[1] Natl Yang Ming Univ, Sch Life Sci, Inst Microbiol & Immunol, Taipei 11221, Taiwan

[2] Natl Yang Ming Univ, Sch Med, Dept Med, Taipei 11221, Taiwan

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 1998年 / 54卷 / 12期

关键词：

boomerang DNA amplification (BDA); capture PCR (CPCR); inverse PCR (IPCR); novel Alu-PCR; panhandle PCR; polymerase chain reaction (PCR); restriction-site PCR (RS-PCR); splinkerette-PCR; targeted gene-walking PCR; vectorette-PCR;

D O I：

10.1007/s000180050262

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many virus and transposon DNAs can integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.

引用

页码：1403 / 1411

页数：9

共 61 条

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