Comparative analysis of 18 sex pheromone plasmids from Enterococcus faecalis: Detection of a new insertion element on pPD1 and implications for the evolution of this plasmid family

被引：34

作者：

Hirt, H ^{[1
]}

Wirth, R ^{[1
]}

Muscholl, A ^{[1
]}

机构：

[1] INST MICROBIOL,D-93053 REGENSBURG,GERMANY

来源：

MOLECULAR & GENERAL GENETICS | 1996年 / 252卷 / 06期

关键词：

sex pheromone plasmids; Enterococcus faecalis; insertion element IS1062; plasmid evolution;

D O I：

10.1007/s004380050272

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new IS element, IS1062, related to the enterococcal IS elements IS6770 and IS1252, was detected in the 3'-terminus of the surface exclusion gene, sep1, of sex pheromone plasmid pPD1 in Enterococcus faecalis. pPD1-bearing cells lack the surface exclusion function, probably as a consequence of this insertion. Analysis of pAD1 and pPD1 sequences (7.5 kb and 2.7 kb, respectively) downstream of their aggregation substance genes revealed no similarity in these DNA regions. Detailed DNA/DNA hybridization studies using DNA probes specific for various pAD1-encoded genes needed for plasmid transfer indicated that the sex pheromone plasmids have evolved by repeated recombination and insertion of diverse transposable elements which presumably account for recent acquisition of antibiotic resistances.

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收藏

页码：640 / 647

页数：8

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