Hybrid nanogels with physical and chemical cross-linking structures as nanocarriers

被引：76

作者：

Morimoto, N

Endo, T

Ohtomi, M

Iwasaki, Y

Akiyoshi, K

机构：

[1] Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan

[2] Toho Univ, Fac Sci, Dept Biomol Sci, Funabashi, Chiba 2748510, Japan

[3] Tokyo Med & Dent Univ, Ctr Excellence Program Frontier Res Mol Destruct, Chiyoda Ku, Tokyo 1010062, Japan

来源：

MACROMOLECULAR BIOSCIENCE | 2005年 / 5卷 / 08期

关键词：

drug delivery systems; microgels; nanoparticles; polysaccharides; protein;

D O I：

10.1002/mabi.200500051

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polymerizable nanogels were prepared by self-assembly of cholesteryl group-bearing pullulan (CHP) with (MPC by radical polymerization in dilute aqueous solution. The solution properties of the polymers in water were investigated by TEM, SEC-MALS, and fluorescence quenching technique. Monodispersed hybrid nanogels of CHPMA-MPC (CM nanogels) (25-30 nm in radius of gyration) were obtained by using CHPMA nanogel as a seed-nanogel. CM nanogels have a dual cross-linking structure that is physically cross-linked with the cholesteryl groups and chemically cross-linked with the MPC polymer chains. CM nanogels trap heat-denatured carbonic anhydrase B (CAB) and prevent their aggregations. The nanogels maintained the ability of trapping and releasing enzymes by host-guest interaction of cholesteryl group and cyclodextrin.

引用

页码：710 / 716

页数：7

共 35 条

[1] Molecular chaperone-like activity of hydrogel nanoparticles of hydrophobized pullulan: Thermal stabilization with refolding of carbonic anhydrase B [J].