Correlation between the expression of the histone H4 mRNA variant H4-v.1 and the levels of histone H4-(86-100) and H4-(89-102) (OGP) in various rat tissues and alveolar macrophages

被引：3

作者：

Poirier, R ^{[1
]}

Lemaire, I ^{[1
]}

Dumont, M ^{[1
]}

Leduc, N ^{[1
]}

Le, HT ^{[1
]}

Lemaire, S ^{[1
]}

机构：

[1] Univ Ottawa, Fac Med, Dept Cellular & Mol Med, Ottawa, ON K1H 8M5, Canada

来源：

PEPTIDES | 2005年 / 26卷 / 08期

关键词：

histone H4 fragments; osteogenic growth peptide; histogranin; peptide processing; H4-v.1 mRNA variant; radioimmunoassay;

D O I：

10.1016/j.peptides.2005.03.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the expression of the osteogenic and antinociceptive C-terminal histone H4-related peptide fragments, H4-(89-102) (OGP) and H4-(86-100), respectively, within various rat tissues and isolated alveolar macrophages (AM) by radioimmunoassay (RIA). OGP was located mainly within the bone marrow, spleen, thymus, and lungs whereas H4-(86-100) was more concentrated within the bone marrow, lymph nodes, spinal cord, pituitaries and thymus. The expression pattern of the two peptides showed similarities with the tissue expression pattern of the histone H4 mRNA variant H4-v.l. In rat AM, OGP and H4-(86-100) levels were significantly stimulated (2.6- and 1.9-fold, respectively) by LPS (1 mu g/ml), along with H4-v.l. mRNA (4.1-fold), but not whole histone H4 (1.1-fold) nor total histone H4 mRNA (1.1-fold). The results suggest that H4-v. I mRNA may play a role in the synthesis of the naturally occurring peptides H4-(86-100) and OGP via the alternative translation product H4-(84-102), but not whole histone H4. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1503 / 1511

页数：9

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