Normoxic wound fluid contains high levels of vascular endothelial growth factor

被引:67
作者
Howdieshell, TR
Riegner, C
Gupta, V
Callaway, D
Grembowicz, K
Sathyanarayana
McNeil, PL
机构
[1] Med Coll Georgia, Dept Surg, Augusta, GA 30912 USA
[2] Med Coll Georgia, Dept Radiol, Augusta, GA 30912 USA
[3] Med Coll Georgia, Dept Anat & Cellular Biol, Augusta, GA 30912 USA
关键词
D O I
10.1097/00000658-199811000-00011
中图分类号
R61 [外科手术学];
学科分类号
摘要
Objective To examine the temporal integration of vascular endothelial growth factor (VEGF), which has been shown to be present in wound fluid, with the putatively related processes of wound fluid oxygen content, wound angiogenesis, and granulation tissue formation. Summary Background Data During cutaneous wound repair, new tissue formation starts with reepithelialization and is followed by granulation tissue formation, including neutrophil and macrophage accumulation, fibroblast ingrowth, matrix deposition, and angiogenesis. Because angiogenesis and increased vascular permeability are characteristic features of wound healing, VEGF may play an important role in tissue repair. Methods A ventral hernia, surgically created in the abdominal wail of female swine, was repaired using silicone sheeting and skin closure. Over time, a fluid-filled wound compartment formed, bounded by subcutaneous tissue and omentum. Ultrasonography was performed serially to examine the anatomy and dimensions of the subcutaneous tissue and wound compartment. Serial wound fluid samples, obtained by percutaneous aspiration, were analyzed for Po-2, Pco(2), pH, and growth factor concentrations. Results Three independent assays demonstrate that VEGF protein is present at substantially elevated levels in a wound fluid associated with the formation of abdominal granulation tissue. However, the wound fluid is not hypoxic at any time. Serial sampling reveals that transforming growth factor beta-1 protein appears in the wound fluid before VEGF. Conclusions The results suggest that VEGF is a prominent regulator of wound angiogenesis and vessel permeability. A factor other than hypoxia, perhaps the earlier appearance of another growth factor, transforming growth factor beta-1, may positively regulate VEGF appearance in the wound fluid.
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页码:707 / 715
页数:9
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