Characterization of psychrotrophic bacteria in the surface and deep-sea waters from the northwestern Pacific Ocean based on 16S ribosomal DNA analysis

被引：27

作者：

Radjasa, OK ^{[1
]}

Urakawa, H ^{[1
]}

Kita-Tsukamoto, K ^{[1
]}

Ohwada, K ^{[1
]}

机构：

[1] Univ Tokyo, Ocean Res Inst, Marine Microbiol Div, Nakano Ku, Tokyo 1648639, Japan

来源：

MARINE BIOTECHNOLOGY | 2001年 / 3卷 / 05期

关键词：

marine psychrotrophs; 16S rRNA; RFLP; vibrionaceae; Vibrio; Photobacterium; Pseudoalteromonas;

D O I：

10.1007/s10126-001-0050-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Seventy-eight 4 degreesC-culturable bacteria were isolated using ZoBell 2216E medium from surface (0-200 m) and deep-sea (1000-9671 m) waters in the northwestern Pacific Ocean. Growth studies indicated that all 4 degreesC-culturable bacteria were psychrotrophs. Six phylotypes were observed in the surface water samples and 8 phylotypes in the deep-sea waters. Phylogenetic characterization based on 16S ribosomal DNA sequence analysis of the representative phylotypes revealed that some bacterial genera, Pseudoalteromas, Photobacterium, and Vibrio, were common to surface and deep-sea waters, and others, Pseudomonas and Halomonas, specifically occurred in surface water. Overall, the members of Vibrionaceae appear to be dominant in both habitats. habitats.

引用

页码：454 / 462

页数：9

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