Species-specificity of rRNA gene transcription in plants manifested as a switch in RNA polymerase specificity

被引:28
作者
Doelling, JH [1 ]
Pikaard, CS [1 ]
机构
[1] WASHINGTON UNIV, DEPT BIOL, ST LOUIS, MO 63130 USA
基金
美国国家科学基金会;
关键词
D O I
10.1093/nar/24.23.4725
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rapid evolution of ribosomal RNA (rRNA) gene promoters often prevents their recognition in a foreign species. Unlike animal systems, we show that foreign plant rRNA gene promoters are recognized in an alien species, but tend to program transcription by a different polymerase. In plants, RNA polymerase I transcripts initiate at a TATATA element (+1 is underlined) important for promoter strength and start-site selection, However, transcripts initiate from +32 following transfection of a tomato promoter into Arabidopsis, The rRNA gene promoter of a more closely related species, Brassica oleracea, programs both +1 and +29 transcription, A paint mutation at +2 improving the identity between the Brassica and Arabidopsis promoters increases +1 transcription, indicating a role for the initiator element in species-specificity Brassica +29 transcripts can be translated to express a luciferase reporter gene, implicating RNA polymerase II, TATA mutations that disrupt TATA-binding protein (TBP) interactions inhibit +29 transcription and luciferase expression, Co-expressed TBP proteins bearing compensatory mutations restore +29 transcription and luciferase activity, suggesting a direct TBP-TATA interaction, Importantly, +1 transcription is unaffected by the TATA mutations, suggesting that in the context of pol I recognition, the TATA-containing initiator element serves a function other than TBP binding.
引用
收藏
页码:4725 / 4732
页数:8
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