Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17

被引：26

作者：

Fedonenko, Yuliya P. ^{[2
]}

Konnova, Olga N. ^{[2
]}

Zdorovenko, Evelina L. ^{[1
]}

Konnova, Svetlana A. ^{[2
]}

Zatonsky, George V. ^{[1
]}

Shashkov, Alexander S. ^{[1
]}

Ignatov, Vladimir V. ^{[2
]}

Knirel, Yuriy A. ^{[1
]}

机构：

[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia

[2] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia

来源：

CARBOHYDRATE RESEARCH | 2008年 / 343卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

lipopolysaccharide; bacterial polysaccharide structure; Azospirillum brasilense; 2-O-methyl-L-rhamnose;

D O I：

10.1016/j.carres.2007.12.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A mixture of two structurally distinct neutral O-polysaccharides was obtained by mild acid degradation of the lipopolysaccharide isolated by the phenol/water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense SIT The following structures of the O-polysaccharides were established by composition and methylation analyses, Smith degradation, and H-1 and C-13 NMR spectroscopy, including a 2D NOESY experiment: [GRAPHICS] where L-Rha2Me stands for 2-O-methyl-L-rhamnose and SHb for the (S)-3-hydroxybutanoyl group. The occurrence of two distinct polysaccharides is reported for the first time in Azospirillum spp. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：810 / 816

页数：7

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