The Role of Posttraumatic Hypothermia in Preventing Dendrite Degeneration and Spine Loss after Severe Traumatic Brain Injury

被引:27
作者
Wang, Chuan-fang [1 ,2 ]
Zhao, Cheng-cheng [1 ,2 ]
Jiang, Gan [3 ]
Gu, Xiao [3 ]
Feng, Jun-feng [1 ,2 ]
Jiang, Ji-yao [1 ,2 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Ren Ji Hosp, Dept Neurosurg, Shanghai 200127, Peoples R China
[2] Shanghai Inst Head Trauma, Shanghai 200127, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Med, Inst Med Sci, Dept Pharmacol, Shanghai 200127, Peoples R China
基金
中国国家自然科学基金;
关键词
CONTROLLED CORTICAL IMPACT; NEUROTROPHIC FACTOR BDNF; NMDA RECEPTOR; MODEL; EXPRESSION; MEMORY; SYNAPTOPHYSIN;
D O I
10.1038/srep37063
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
Posttraumatic hypothermia prevents cell death and promotes functional outcomes after traumatic brain injury (TBI). However, little is known regarding the effect of hypothermia on dendrite degeneration and spine loss after severe TBI. In the present study, we used thy1-GFP transgenic mice to investigate the effect of hypothermia on the dendrites and spines in layer V/VI of the ipsilateral cortex after severe TBI. We found that hypothermia (33 degrees C) dramatically prevented dendrite degeneration and spine loss 1 and 7 days after CCI. The Morris water maze test revealed that hypothermia preserved the learning and memory functions of mice after CCI. Hypothermia significantly increased the expression of the synaptic proteins GluR1 and PSD-95 at 1 and 7 days after CCI in the ipsilateral cortex and hippocampus compared with that of the normothermia TBI group. Hypothermia also increased cortical and hippocampal BDNF levels. These results suggest that posttraumatic hypothermia is an effective method to prevent dendrite degeneration and spine loss and preserve learning and memory function after severe TBI. Increasing cortical and hippocampal BDNF levels might be the mechanism through which hypothermia prevents dendrite degeneration and spine loss and preserves learning and memory function.
引用
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页数:12
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