A rapid method for determining dynamic binding capacity of resins for the purification of proteins

被引：60

作者：

Do, Tri ^{[1
]}

Ho, Frank ^{[1
]}

Heidecker, Bettina ^{[2
]}

Witte, Krista ^{[2
]}

Chang, Lucille ^{[1
]}

Lerner, Laura ^{[1
]}

机构：

[1] Raven Biotechnol Inc Mfg, San Francisco, CA 94080 USA

[2] ForteBio Inc, Menlo Pk, CA 94025 USA

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2008年 / 60卷 / 02期

关键词：

dynamic binding capacity; chromatography; protein purification; biolayer interferometry; light diffraction; protein assay;

D O I：

10.1016/j.pep.2008.04.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Biolayer interferometry is a novel method for quantifying macromolecules, such as proteins, in solution. The presence of other, non-binding molecules does not interfere with quantification, which allows one to measure the concentration of the molecule of interest in a crude mixture. Here we apply this method to determining the dynamic binding capacity of affinity resins. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：147 / 150

页数：4

共 8 条

[1]

Identification of protein A media performance attributes that can be monitored as surrogates for retrovirus clearance during extended re-use [J].