Efficient transfection of DNA by liposomes formulated with cationic gemini amphiphiles

被引：70

作者：

Bombelli, C

Faggioli, F

Luciani, P

Mancini, G

Sacco, MG

机构：

[1] Univ Roma La Sapienza, CNR, Ist Metodol Chim, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

[3] CNR, Ctr Eccellenza Mat Innovativi Nanostrutturati App, I-20090 Milan, Italy

[4] CNR, Inst Tecnol Biomed, I-20090 Milan, Italy

来源：

JOURNAL OF MEDICINAL CHEMISTRY | 2005年 / 48卷 / 16期

关键词：

D O I：

10.1021/jm050477r

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Cationic liposomes formulated with neutral 1,2-dimyristoyl-sn-glycero-3-phosphocholine and cationic gemini surfactants were used for transfecting different cell lines with a reporter gene. The efficiency in the transfection has been correlated to the high extent of DNA condensation observed by circular dichroism, condensation shown to depend heavily on the gemini spacer structure. Transfection efficiency was better than that obtained with a commercial lipofection kit.

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页码：5378 / 5382

页数：5

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