N-Acyl-homoserine Lactones from Enterobacter sakazakii (Cronobacter spp.) and Their Degradation by Bacillus cereus Enzymes

被引：15

作者：

Araujo, Francisca Diana da Silva ^{[1
]}

Ramires Esper, Luciana Maria ^{[2
]}

Kuaye, Arnaldo Yoshiteru ^{[2
]}

Sircili, Marcelo Palma ^{[3
]}

Marsaioli, Anita Jocelyne ^{[1
]}

机构：

[1] Univ Estadual Campinas, Inst Chem, BR-13084971 Campinas, SP, Brazil

[2] Univ Estadual Campinas, Dept Food Technol, Fac Food Engn, BR-13084971 Campinas, SP, Brazil

[3] Butantan Inst, Bacteriol Lab, BR-05503900 Sao Paulo, Brazil

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2012年 / 60卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

Enterobacter sakazakii (Cronobacter spp.); Bacillus cereus; N-acyl-homoserine lactones; foodborne diseases; enzymes; QUORUM-SENSING SIGNALS; BIOFILM FORMATION; COMMUNICATION;

D O I：

10.1021/jf203846f

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

A chemical study of acyl-homoserine lactones (acyl-HSLs) produced by Enterobacter sakazakii resulted in the identification of three molecules: (S)-N-heptanoyl-HSL, (S)-N-dodecanoyl-HSL and (S)-N-tetradecanoyl-HSL. Mixed cultures of E. sakazakii and Bacillus cereus depleted E. sakazakii acyl-HSLs, suggesting acyl-HSL degradation by B. cereus hydrolases (hydrolysis of the lactone or amide moiety). The expression of B. cereus acyl-HSL lactonase and acyl-homoserine acylase was confirmed by monitoring the biotransformation of (S)-N-dodecanoyl-HSL into (S)-N-dodecanoyl-homoserine, dodecanoic acid and homoserine in the presence of B. cereus whole cells, using electrospray-mass spectrometry (ESI-MS).

引用

页码：585 / 592

页数：8

共 32 条

[1]

[Anonymous], 2002, Oxidative Phosphorylation

[2]

BACILLUS-CEREUS IN INFANT FOODS AND DRIED MILK-PRODUCTS [J].

BECKER, H ;

SCHALLER, G ;

VONWIESE, W ;

TERPLAN, G .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1994, 23 (01) :1-15

[3]

Bennett R.W., 2001, COMPENDIUM METHODS M, P311

[4]

Invasive Enterobacter sakazakii disease in infants [J].

Bowen, Anna B. ;

Braden, Christopher R. .

EMERGING INFECTIOUS DISEASES, 2006, 12 (08) :1185-1189

[5]

Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria [J].

Cha, C ;

Gao, P ;

Chen, YC ;

Shaw, PD ;

Farrand, SK .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (11) :1119-1129

[6]

Czajkowski R, 2009, ACTA BIOCHIM POL, V56, P1

[7]

Dong YH, 2005, J MICROBIOL, V43, P101

[8]

Identification of quorum-quenching N-acyl homoserine lactonases from Bacillus species [J].

Dong, YH ;

Gusti, AR ;

Zhang, Q ;

Xu, JL ;

Zhang, LH .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (04) :1754-1759

[9]

Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase [J].

Dong, YH ;

Wang, LH ;

Xu, JL ;

Zhang, HB ;

Zhang, XF ;

Zhang, LH .

NATURE, 2001, 411 (6839) :813-817

[10]

A case of fatal neonatal Bacillus cereus meningitis [J].

Evreux, F. ;

Delaporte, B. ;

Leret, N. ;

Buffet-Janvresse, C. ;

Morel, A. .

ARCHIVES DE PEDIATRIE, 2007, 14 (04) :365-368

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