The effect of growth conditions on in vitro adherence, invasion, and NAF expression by Proteus mirabilis 7570

被引：10

作者：

Latta, RK ^{[1
]}

Schur, MJ ^{[1
]}

Tolson, DL ^{[1
]}

Altman, E ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1998年 / 44卷 / 09期

关键词：

adhesion; invasion; nonagglutinating fimbriae; Proteus mirabilis;

D O I：

10.1139/cjm-44-9-896

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteus mirabilis is a common cause of upper urinary tract infections. Fimbriae-mediated adherence of this organism to urinary tract epithelium and invasion of host cells are factors thought to be important in its pathogenesis. We have assessed the effect of growth in serum, blood, and urine on the ability of P. mirabilis 7570 to adhere to and invade in vitro the cell line EJ/28, derived from a human urinary tract tumour, and to express nonagglutinating fimbriae (NAF). Proteus mirabilis was capable of adhering to EJ/28 cells to varying degrees depending upon the growth conditions used. It was invasive under all conditions, except when grown in urine, and was found to be particularly so when serum or blood was present in the media. Expression of NAF occurred under all growth conditions examined and was Limited only by a decrease in temperature.

引用

页码：896 / 904

页数：9

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