Protective effect and cytokine production of a Lactobacillus plantarum strain isolated from ewes' milk cheese

被引:21
作者
Haza, AI [1 ]
Zabala, A [1 ]
Morales, P [1 ]
机构
[1] Univ Complutense Madrid, Fac Vet, Dept Nutr & Bromatol 3, Madrid 28040, Spain
关键词
Lb. plantarum CBL/J; inhibition of the cellular viability; N-nitrosamines; cytokines;
D O I
10.1016/S0958-6946(03)00146-8
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Ten lactic acid bacteria strains were isolated from a rippened ewes' milk cheese and tested for stimulation or inhibition of the viability of Vero and myeloma cells. None of the strains significantly affected the viability of Vero cells. However, an isolate (CBL/J) showed a strong inhibition of the cellular viability (10(8) cfu mL(-1), 10.1% of survival on myeloma cells). Isolate CBL/J was submitted to a characterisation scheme and identified as Lactobacillus plantarum by DNA sequencing of 16S rRNA Polymerase Chain Reaction (PCR) products. Lb. plantarum CBL/J showed a protective effect against three N-nitrosamines [N-nitrosopyrrolidine (NPYR), N-nitrosodibuthylamine (NDBA) and N-nitrosopiperidine (NPIP)] but not against N-nitrosodimethylamine (NDMA). The highest protective effect was observed against NPIP at populations of 10(7) cfu mL-' (110.9% of survival on Vero cells). To test the effect of Lb. plantarum CBL/J on cytokine production, [tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin-8 (IL-8)], the human macrophage cell line (THP-1) was cultured in the presence and absence of lipopolysaccharide (LPS). Lb. plantarum CBL/J induced IL-1beta release when cells were stimulating with and without LPS. However, IL-8 production was not observed in the presence of LPS and TNF-alpha release was only produced in the presence of the LPS. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:29 / 38
页数:10
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