Unexpected failure rates for modular assembly of engineered zinc fingers

被引：272

作者：

Ramirez, Cherie L. ^{[1
,2
,3
]}

Foley, Jonathan E. ^{[1
,2
]}

Wright, David A. ^{[4
]}

Muller-Lerch, Felix ^{[5
]}

Rahman, Shamim H. ^{[5
]}

Cornu, Tatjana I. ^{[5
]}

Winfrey, Ronnie J. ^{[4
]}

Sander, Jeffry D. ^{[4
,6
]}

Fu, Fengli ^{[4
,6
]}

Townsend, Jeffrey A. ^{[4
]}

Cathomen, Toni ^{[5
]}

Voytas, Daniel F. ^{[4
]}

Joung, J. Keith ^{[1
,2
,3
,7
]}

机构：

[1] Massachusetts Gen Hosp, Ctr Canc Res, Mol Pathol Unit, Charlestown, MA 02129 USA

[2] Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Charlestown, MA 02129 USA

[3] Harvard Univ, Sch Med, Div Med Sci, Biol & Biomed Sci Program, Boston, MA 02115 USA

[4] Iowa State Univ, Dept Genet Dev & Cell Biol, Roy J Carver Co Lab 1035A, Ames, IA 50011 USA

[5] Charite Univ Med Berlin, Inst Virol, D-12203 Berlin, Germany

[6] Iowa State Univ, Interdept Grad Program Bioinformat & Computat Bio, Ames, IA 50011 USA

[7] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 05期

关键词：

D O I：

10.1038/nmeth0508-374

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：374 / 375

页数：3

共 6 条

[1] Human zinc fingers as building blocks in the construction of artificial transcription factors [J].

Bae, KH ;

Do Kwon, Y ;

Shin, HC ;

Hwang, MS ;

Ryu, EH ;

Park, KS ;

Yang, HY ;

Lee, D ;

Lee, Y ;

Park, J ;

Kwon, HS ;

Kim, HW ;

Yeh, BI ;

Lee, HW ;

Sohn, SH ;

Yoon, J ;

Seol, W ;

Kim, JS .

NATURE BIOTECHNOLOGY, 2003, 21 (03) :275-280

[2] Validated zinc finger protein designs for all 16 GNN DNA triplet targets [J].

Liu, Q ;

Xia, ZQ ;

Case, CC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (06) :3850-3856

[3] Zinc finger tools: custom DNA-binding domains for transcription factors and nucleases [J].

Mandell, Jeffrey G. ;

Barbas, Carlos F., III .

NUCLEIC ACIDS RESEARCH, 2006, 34 :W516-W523

[4] Gene targeting using zinc finger nucleases [J].

Porteus, MH ;

Carroll, D .

NATURE BIOTECHNOLOGY, 2005, 23 (08) :967-973

[5] Evaluation of a modular strategy for the construction of novel polydactyl zinc finger DNA-binding proteins [J].

Segal, DJ ;

Beerli, RR ;

Blancafort, P ;

Dreier, B ;

Effertz, K ;

Huber, A ;

Koksch, B ;

Lund, CV ;

Magnenat, L ;

Valente, D ;

Barbas, CF .

BIOCHEMISTRY, 2003, 42 (07) :2137-2148

[6] Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly [J].

Wright, David A. ;

Thibodeau-Beganny, Stacey ;

Sander, Jeffry D. ;

Winfrey, Ronnie J. ;

Hirsh, Andrew S. ;

Eichtinger, Magdalena ;

Fu, Fengli ;

Porteus, Matthew H. ;

Dobbs, Drena ;

Voytas, Daniel F. ;

Joung, J. Keith .

NATURE PROTOCOLS, 2006, 1 (03) :1637-1652

← 1 →