Temperature dependent intermediate structures during the main phase transition of dimyristoyl phosphatidylcholine vesicles a combined iodine laser-temperature jump and time resolved cryo-electron microscopy study

被引：10

作者：

Groll, R ^{[1
]}

Bottcher, A ^{[1
]}

Jager, J ^{[1
]}

Holzwarth, JF ^{[1
]}

机构：

[1] MAX PLANCK GESELL, FRITZ HABER INST, D-14195 BERLIN, GERMANY

来源：

BIOPHYSICAL CHEMISTRY | 1996年 / 58卷 / 1-2期

关键词：

dimyristoyl phosphatidylcholine vesicles; lipid-phase transition; laser temperature jump; cryo-electron microscopy; vitrified aqueous lamella;

D O I：

10.1016/0301-4622(95)00085-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetics of the main phase transition of dimyristoylphosphatidyl choline (DMPC) unilamellar vesicles were investigated in the time range from microseconds to seconds. Iodine laser-temperature jump (ILTJ) experiments showed three discrete relaxation phenomena. Time resolved cryo-electron microscopy (GEM) was applied to produce images of intermediate states typical for the relaxation times of lipid vesicles in the micro- to millisecond time window, A careful measurement of the rate of temperature decrease observed during the production of vitrified lamellae of aqueous samples on a copper grid was performed. The best conditions resulted in average rates of cooling of 3 x 10(4) K/s. By comparing the images from CEM of DMPC vesicle samples vitrified above, at, and below the phase transition temperature a structural model was designed, which explains the temperature jump relaxation times in the micro- to millisecond time range by the formation and disappearance of coexisting clusters of crystalline, intermediate, and fluid lipid areas inside the DMPC bilayers.

引用

页码：53 / 65

页数：13

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